Fats may limit cell organism size in carbon-rich media

lipids

Bacterial cells in carbon-rich media (purple and blue) grow twice as big as those in carbon-poor media (green). New research shows they can grow big, however, only if they can make fats with the carbon.

Fat (lipids) limits how big bacterial cells can be. “If you prevent cells from making fat, they’re smaller, and if you give them extra fat or allow them to make more fat, they get bigger,” said Levin, professor of biology in Arts & Sciences. “Fat makes cells fat.”

“If we hit the cells with an antibiotic that targets fatty-acid synthesis, we really saw a significant drop in cell size” Vadia said.

Also, by turning up FadR, a transcription factor that activates expression of the fatty-acid synthesis genes, the cells got bigger.

“It doesn’t seem to matter what the lipids are, really,” Levin said, “provided you have enough of them. We found we could give the cells oleic acid, a fat found in avocados and olive oil, to supplement diminished fatty-acid synthesis and as long as the added fatty acid got into the membrane, the cells could recover.”

A little place for my stuff | EurekAlert! Science News

Fatty Acid Availability Sets Cell Envelope Capacity and Dictates Microbial Cell Size: Current Biology


Hydrogenation: transform liquid oil into solid fat
Applied Science

Olive Oil Did WHAT to my Triglycerides??!!?? (Pt 2)

Advertisements

The importance of beneficial plant partners

When a plant is introduced (accidentally or intentionally, but usually by humans) into a new region, many factors can influence the ability for that species to become established. One major factor at play is the different set of species it will interact with in this new environment—will they work with it or against it? We naturally tend to focus on the negatives; like whether there are enemies like pathogens, predators or other competitors that will control it.

What has received less attention is how positive interactions are affecting the spread of non-native species. For example, we know that the availability of pollinators is important for many plant species. So when a species moves, it doesn’t just leave its enemies behind, it also leaves its friends, its beneficial partnerships.

And it appears that for symbiotic legumes, these beneficial partners matter a lot. Their associated rhizobia matter so much that we can see their impacts on legume species spread at a global scale, across multiple continents and islands.

Read more: Legumes’ microbe relationships hold them back from travelling the globe – ECOS

Microbial communities affected by type of carbon “food” sources

carbontypes.png

A new study has found that:

The type of carbon source affects not only the composition and activity of natural microbial communities, but also in turn the types of mineral products that form in their environment.

“We’ve illustrated that as microorganisms alter their environment, their environment then affects the type of microorganisms that are there and their activity.”

Researchers took anaerobic respiration microbial communities and presented them with one of three carbon sources: glucose, a six-carbon sugar; lactate, a four-carbon compound; or acetate, a simple two-carbon compound.

Their analysis showed that a distinct series of changes occurred consistently when microbes were exposed to lactate or acetate-rich environments. However, in glucose-rich environments, they observed varying patterns of changes.

“We think that, because glucose is a larger, more complex compound that can be broken down into many simpler compounds, this opens up more chemical pathways in the community through which it can be used, and that this diverse metabolic potential accounts for the different patterns we’re seeing,” said O’Loughlin.

Impact of Organic Carbon Electron Donors on Microbial Community Development under Iron- and Sulfate-Reducing Conditions

When dogs come to visit the Barefoot

I normally walk barefoot all over this property. Over the years I’ve learnt to deal with rocks, thistles, baking concrete, and soil living spiders. However there’s one aspect of it that I’m yet to find a solution for. Dog shit.

Land mines.

We have two dogs visiting at the moment and one has had diarrhoea. It’s bad enough dodging the land mines but when it comes out runny its a whole new field game.

While washing my feet, I noticed that they could probably use a good beach sand scrubbing. One of the downsides of going barefoot is that you become complacent. It’s just so convenient that you don’t even think twice before heading in or out of the house and because I’m so tall, I rarely even see my feet. Sometimes wandering in and out carries with it soil from the garden, and I’ve noticed that while despite regular showers dead skin that would normally get rubbed off when wearing shoes, can stay and become food for microbes. Particularly on the tops of toes. Nothing a good brushing doesn’t clean off but interesting none the less, just not something I’ve had to deal with before.

I probably should wash my feet more than once in a blue moon.

Update:

I woke in the middle of the night to commotion upstairs, walking up the staircase barefoot in dim light, no sooner had I stepped off the stair mat… ugh. It’s all over the house. I don’t think I could own a dog and a house with carpet at the same time…

Floorboards, I love you. It took longer to clean the mats and rugs than the entire floor.

Now I’m playing a game of is the smell still here or there? Did I miss something? Ugh. To make matters worse tomorrow is 37C and it needs to be clean or it’ll stink the place up when my younger brother’s young son comes to visit and does his usual trick of face planting into the floor.

I found the smell. It was on the back of my calf muscle. Shit happens.